Seminar in Department of Chemistry: Development of fluorogenic bio-application based on supramolecular interactions

Dr. Kalyan K. Sadhu, Institut de Science et d’Ingénierie Supramoléculaires Université de Strasbourg, France will visit the Department of Chemistry. He will deliver a talk on 07th March 2013 at 3:00 pm in L3. 
 
Title of talk: Development of fluorogenic bio-application based on supramolecular interactions 
 
Abstract :-
 
The photoactivatable probes overcome the limitations of fluorescent proteins in live cell imaging. A genetically modified version of class A type b-lactamase has been used as the tag protein (BL-tag). The swiftness of the protein labeling strategy was developed on the basis of the aggregation-elimination based probe. This new straightforward technique for protein labeling was applicable for a versatile emissive range of fluorophores in live cell imaging. The synthesis of next-generation probe with photoinduced electron transfer process showed effective fast kinetics. The programmability of oligonucleotide recognition offers an attractive platform to direct the reactive partner that can engage in chemical reactions. For nucleic acid templated reactions, photoreduction of azide-based immolative linker by catalytic Ru(II) conjugates to uncage rhodamine was achieved in response to DNA template or even cellular RNA. The same Ru(II) based protocol was applied to different oligomeric protein templates in live cancer cells and bacteria. The generality of the approach was validated with three sets of ligand based on biotin, desthiobiotin and raloxifene.
 
All are cordially invited to the seminar. Tea will be provided after the seminar at Fuel-Zap.
English
Dates: 
Thursday, 7 March, 2013 - 15:00